BBa_K337056

BBa_K337056 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K337056
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rebecca Berrens
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:12:06

synthetic binding site of miR 122 (imperfect) KD: 64%



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K337056_sequence (Version 1)

Description

imperfect binding site of miR122 in the tuning construct leads to a knockdown percentage of 64% percent if introduced into the 3'UTR of the gene of interest (GOI). part can be either co-transfected with a synthetic miRNA or transfected into liver cell lines (i.e. Huh7).

Notes

designed to lead to 60% KD if introduced into the 3'UTR of your GOI and transfected into liver cell lines or co-transfected with miR122

Source

synthetic

Sequence Annotation Location Component / Role(s)
misc
18,25
feature/misc sequence_feature
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K337056/1