BBa_K337056

BBa_K337056 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K337056
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rebecca Berrens
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:12:06

synthetic binding site of miR 122 (imperfect) KD: 64%



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_K337056_sequence (Version 1)

    Description

    imperfect binding site of miR122 in the tuning construct leads to a knockdown percentage of 64% percent if introduced into the 3'UTR of the gene of interest (GOI). part can be either co-transfected with a synthetic miRNA or transfected into liver cell lines (i.e. Huh7).

    Notes

    designed to lead to 60% KD if introduced into the 3'UTR of your GOI and transfected into liver cell lines or co-transfected with miR122

    Source

    synthetic

    Sequence Annotation Location Component / Role(s)
    misc
    		18,25
    		feature/misc sequence_feature

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K337056/1
     

    Attachments

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