Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rebecca Berrens
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:12:06
synthetic binding site of miR 122 (imperfect) KD: 24%
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K337057_sequence (Version 1) |
Description
imperfect binding site of miR122 in the tuning construct leads to a knockdown percentage of 64% percent if introduced into the 3'UTR of the gene of interest (GOI). part can be either co-transfected with a synthetic miRNA or transfected into liver cell lines (i.e. Huh7).Notes
designed to lead to 30% KD if introduced into the 3'UTR of your GOI and transfected into liver cell lines or co-transfected with miR122.Source
synthetic| Sequence Annotation | Location | Component / Role(s) |
| seed miR122 seed mismatch | 15,22 15,15 | sequence_feature feature/misc sequence_feature feature/misc |