Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lucas Hartsough
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:12:07
PHA Synthase Composite, Part 2/2
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K338004_sequence (Version 1) |
Description
This is half of a planned part which would contain all three PHA synthase genes required to produce polyhydroxybutyrate (PHB) in cells: phaA, phaB1, phaC1. This half contains phaB1 and phaC1:Notes
When ligated downstream of [[Team:Caltech/BBa_K338003|BBa_K338003]], the completed construct was designed to express all three PHA synthase genes required to make PHB oligomers from soybean oil. The three genes would be transcribed polycistronically on a single mRNA transcript under the IPTG-inducible control of theNote that these three genes should only cause the production of PHB oligomers in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the phaC1 gene could cause some crosslinking, but this has not been experimentally verified.
Source
Registry of Standard Biological Parts| Access | Instance | Definition |
| public public public public | BBa_K156013 BBa_B0034 BBa_K156014 BBa_B0015 | Bioplastic BBa_B0034 Bioplastic BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K156013 BBa_B0034 BBa_K156014 BBa_B0015 | 1,741 750,761 768,2116 2125,2253 | Bioplastic BBa_B0034 Bioplastic BBa_B0015 |