BBa_K341000

BBa_K341000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K341000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Danyang He

Date created: 2010-10-17 11:00:00

Date modified: 2015-05-08 01:12:07

recombination sequence + eGFP+I-Sce I cutting site

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_K341000_sequence (Version 1)

Description

The backbone of our part BBa-K341000_eGFP_recombination is PSB1C13, resistant to Amp. Restriction enzyme I-SceI cutting site, Recombination sequence 1, homologous to part of modified E.coli genome, coding sequence of gene eGFP, recombination sequence 2, homologous to another part of modified E.coli genome, I-Scel cutting site( the elements detailed above are listed in the order from 5??? to 3???) are added into the backbone with EcoR I and Pst I. The biobrick we construct in this part is to verify landing pad method, on which our project is based on.

Source

From plasmid containing gene eGFP.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
I-Sce I cutting site	11,26	feature/misc, sequence_feature
eGFP	43,759	feature/cds, CDS
recombination sequence 1	43,67	feature/misc, sequence_feature
recombination sequence 2	739,759	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K341000/1

Member of these Collections

• iGEM Parts Registry