Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danyang He
Date created: 2010-10-17 11:00:00
Date modified: 2015-05-08 01:12:07
recombination sequence + eGFP+I-Sce I cutting site
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K341000_sequence (Version 1) |
Description
The backbone of our part BBa-K341000_eGFP_recombination is PSB1C13, resistant to Amp. Restriction enzyme I-SceI cutting site, Recombination sequence 1, homologous to part of modified E.coli genome, coding sequence of gene eGFP, recombination sequence 2, homologous to another part of modified E.coli genome, I-Scel cutting site( the elements detailed above are listed in the order from 5??? to 3???) are added into the backbone with EcoR I and Pst I. The biobrick we construct in this part is to verify landing pad method, on which our project is based on.Source
From plasmid containing gene eGFP.| Sequence Annotation | Location | Component / Role(s) |
| I-Sce I cutting site eGFP recombination sequence 1 recombination sequence 2 | 11,26 43,759 43,67 739,759 | feature/misc sequence_feature feature/cds CDS feature/misc sequence_feature feature/misc sequence_feature |