Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Teng Li
Date created: 2010-10-20 11:00:00
Date modified: 2015-05-08 01:12:08
Kanamycin resistance(with Promoter) + I-sceI site+ Recombination site
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K341456_sequence (Version 1) |
Description
Kanamycin resistance(with Promoter) + I-sceI site+ Recombination site+DraIII cutting site.This part can be used as a recombination unit between a plasmid and the chromosome in the "In-vivo Recombination System" in iGEM project "Tsinghua 2010"
Notes
This part involves DraIII cutting site at two ends of the unit, this cutting site is compatible with BBF RFC 61Source
Kanamycin resistance gene is form plasmid "pKD13"| Sequence Annotation | Location | Component / Role(s) |
| Km Resistance gene Recombination site Recombination site Dra III cutting site Dra III cutting site I-sceI cutting site I-sceI cutting site promoter | 68,862 33,57 1230,1254 1273,1281 6,14 15,32 1255,1272 863,1229 | CDS feature/cds feature/dna sequence_feature feature/dna sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/promoter promoter |