Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ozkan IS
Date created: 2010-10-01 11:00:00
Date modified: 2015-05-08 01:12:11
pCooF Promoter from Rhodospirillum rubrum
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K352002_sequence (Version 1) |
Description
CooA is a CO-sensing protein that activates the transcription of genes encoding the CO-oxidation (coo) regulon, whose polypeptide products are required for utilizing CO as an energy source in Rhodospirillum rubrum. CooA binds to a position overlapping the -35 element of the P(cooF) promoter, similar to the arrangement of class II CRP (cAMP receptor protein)- and FNR (fumarate and nitrate reductase activator protein)-dependent promoters when expressed in Escherichia coli.The CO-dependent anaerobic growth of Rhodospirillum rubrum relies on a CO oxidation system encoded by two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ. The key products of the coo regulon are an O2-
sensitive CO dehydrogenase (CooS), a CooS-associated Fe-S protein (CooF), and a CO-tolerant hydrogenase (CooH), and the expression of the genes depends upon the activity of the CooA protein, which senses CO under anaerobic conditions.
CO-independent basal level transcription of PcooF is not detectable due to the absence of active CooA.
Notes
The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.Source
S.W. Singer, M.B. Hirst and P.W. Ludden, CO-dependent H2 evolution by Rhodospirillum rubrum: role of CODH:CooF complex, Biochim Biophys Acta - Bioenerg 1757 (2006), pp. 1582???1591| Sequence Annotation | Location | Component / Role(s) |
| A+T rich sequence CooA binding site CooA binding site -10 Region Transcription Start Protected by CooA Protected by CooA+RNAP | 8,33 41,44 52,56 79,84 92,93 36,61 23,91 | sequence_feature feature/misc feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/misc sequence_feature feature/start start_codon feature/conserved conserved_region conserved_region feature/conserved |