Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Selin Akkuzu
Date created: 2010-10-01 11:00:00
Date modified: 2015-05-08 01:12:11
C4G Mutated pCooF From Rhodospirillum rubrum
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K352005_sequence (Version 1) |
Description
This part is the mutated version of pCooF promoter. The mutation involves conversion cytosine to guanine at fourth base. The binding strength of pCooF to CooA is expected to diminish.CooA is a CO-sensing protein that activates the transcription of genes encoding the CO-oxidation (coo) regulon, whose polypeptide products are required for utilizing CO as an energy source in Rhodospirillum rubrum. CooA binds to a position overlapping the 35 element of the P(cooF) promoter, similar to the arrangement of class II CRP (cAMP receptor protein)and FNR (fumarate and nitrate reductase activator protein)-dependent promoters when expressed in Escherichia coli.
The CO-dependent anaerobic growth of Rhodospirillum rubrum relies on a CO oxidation system encoded by two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ. The key products of the coo regulon are an O2- sensitive CO dehydrogenase (CooS), a CooS-associated Fe-S protein (CooF), and a CO-tolerant hydrogenase (CooH), and the expression of the genes depends upon the activity of the CooA protein, which senses CO under anaerobic conditions.
CO-independent basal level transcription of PcooF is not detectable due to the absence of active CooA.
Notes
The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.Source
De Vooght KM, van Wijk R, van Solinge WW. Management of gene promoter mutations in molecular diagnostics. Clin. Chem.55(4),698???708 (2009).| Sequence Annotation | Location | Component / Role(s) |
| CooA Binding Side CooA Binding Side Transcription Start Side -10 Region A+T Rich Sequence Protected by CooA+RNAP Protected by CooA | 41,44 52,58 92,93 79,86 8,33 23,91 36,61 | feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding feature/start start_codon feature/misc sequence_feature feature/misc sequence_feature feature/conserved conserved_region conserved_region feature/conserved |