BBa_K354000

BBa_K354000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K354000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gregory Meyer
Date created: 2010-10-18 11:00:00
Date modified: 2015-05-08 01:12:11

Lac/Ara-1 IPTG Inducible Promoter



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K354000_sequence (Version 1)

Description

Derived from the L-arabinose and Lactose operons, this synthetic promoter remains in the 'off' state due to the repression of the AraC protein. AraC is constitutively expressed in bacteria possessing the arabinose operon and as such is present in E. coli. When exogenous arabinose and/or IPTG is added, the AraC protein is freed from the promoter thus allowing RNA polymerase to bind and initiate transcription. Hence, addition of arabinose or IPTG will switch the promoter into the 'on' state.

Notes

The affiliation between AraC and its binding sequence had to be ensured so as to prevent basal expression and false triggering of the promoter.

Source

The promoter was synthesised via primer extension.

Sequence Annotation Location Component / Role(s)
-33 Hexamer
AraC Binding Site
Symmetrical Synthetic Operator
-10 Hexamer
Lac Operon operator Sequence
38,43
2,37
44,57
58,66
72,102
promoter feature/promoter
non_covalent_binding_site feature/binding
operator feature/operator
promoter feature/promoter
feature/operator operator
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
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synbiohub#topLevel
BBa_K354000/1