Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gregory Meyer
Date created: 2010-10-19 11:00:00
Date modified: 2015-05-08 01:12:11
PlcR Promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K354003_sequence (Version 1) |
Description
The PlcR promoter is positively regulated by the PlcR-PapR fusion peptide and is repressed by the SpooA peptide. Derived from Bacillus thuringiensis, the PlcR promoter regulates the expression of a host of genes, one of which is the PlcR gene itself. Thus there exists a positive-feedback loop controlling the activation of the operon. Within the promoter are clearly defined binding regions for both the PlcR-PapR fusion peptide as well as the SpooA repressor protein.Notes
So as to create a library of promoters with differing transcriptional activities, randomised nucleotides were introduced into the -10 box - the binding site for the RNAP sigma-factor.Source
The sequence is derived from the PlcR promoter in Bacillus thuringiensis and was synthesized via primer extension.| Sequence Annotation | Location | Component / Role(s) |
| Sigma A binding site PlcR binding site Transcriptional start site SpoOA binding site SpoOA binding site | 64,69 27,43 75,75 9,15 51,57 | non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding start_codon feature/start non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding |