CqsA

BBa_K356001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K356001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: C David Owen, James Taylor

Date created: 2010-08-31 11:00:00

Date modified: 2015-05-08 01:12:11

CAI-1 Generator

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K356001_sequence (Version 1)

Description

Vibrio cholerae CqsA with strong constituitive promotor, ribosome binding site and terminator. Generates CAI-1, a V. cholerae specific autoinducer which is detected by CqsS.

Notes

CqsA codon optimized by Mr Gene. Biobrick scars were placed between all parts as would be produced by standard assembly.

Source

The CqsA coding reigon (K356000) comes from V. cholerae genomic sequence (NCBI database) synthesized by GeneArt. The rest of the subparts are from the registry.

Components

Access	Instance	Definition
public	BBa_B0034	BBa_B0034
public	BBa_J23100	BBa_J23100
public	BBa_K356000	CqsA
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	1,12	BBa_B0034
BBa_J23100	21,55	BBa_J23100
BBa_K356000	64,1486	CqsA
BBa_B0015	1495,1623	BBa_B0015

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K356001/1

Member of these Collections

• //cds
• iGEM Parts Registry