Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anish Kapadia
Date created: 2010-06-22 11:00:00
Date modified: 2015-05-08 01:12:11
muconate and chloromuconate cycloisomerase
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K357001_sequence (Version 1) |
Description
Muconate Lactonizing Enzyme (MLE), an homooctameric enzyme, catalyses the conversion of cis,cis-muconate (CCM) to muconolactone (ML) in the catechol branch of the beta-ketoadipate pathway. This pathway is used in soil microbes to breakdown lignin-derived aromatics, catechol and protocatechuate, to citric acid cycle intermediates. Some bacterial species are also capable of dehalogenating chloroaromatic compounds by the action of chloromuconate lactonizing enzymes (Cl-MLEs). MLEs are members of the enolase superfamily characterized by the presence of an enolate anion intermediate which is generated by abstraction of the alpha-proton of the carboxylate substrate by an active site residue and that is stabilized by coordination to the essential Mg2+ ion.Notes
The sequence was changed from the original to remove a PstI restriction site located around bp 124, to remove the restriction site while maintaining a conserved aa sequence.Source
AUTHORS Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D.,Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,
Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,
Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,
Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,
Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,
Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,
Duesterhoft,A., Tummler,B. and Fraser,C.
TITLE Complete genome sequence and comparative analysis of the
metabolically versatile Pseudomonas putida KT2440
JOURNAL Environ. Microbiol. 4 (12), 799-808 (2002)
| Sequence Annotation | Location | Component / Role(s) |
| Start Codon Stop Codon | 1,3 1120,1122 | feature/start start_codon feature/stop stop_codon |