Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anish Kapadia
Date created: 2010-06-22 11:00:00
Date modified: 2015-05-08 01:12:11
muconolactone Delta-isomerase or muconolactone D-isomerase
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K357002_sequence (Version 1) |
Description
The muconolactone Delta-isomerase (EC 5.3.3.4) is an enzyme that catalyzes the chemical reaction which converts (S)-5-oxo-2,5-dihydrofuran-2-acetate to 5-oxo-4,5-dihydrofuran-2-acetate. This enzyme belongs to the family of isomerases, specifically those intramolecular oxidoreductases transposing C=C bonds. The systematic name of this enzyme class is 5-oxo-4,5-dihydrofuran-2-acetate Delta3-Delta2-isomerase. This enzyme is also called muconolactone isomerase. This enzyme participates in benzoate degradation via hydroxylation.Notes
The sequence was changed from the original to remove a PstI restriction site located around bp 104, to remove the restriction site while maintaining a conserved aa sequence.Source
AUTHORS Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D.,Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,
Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,
Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,
Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,
Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,
Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,
Duesterhoft,A., Tummler,B. and Fraser,C.
TITLE Complete genome sequence and comparative analysis of the
metabolically versatile Pseudomonas putida KT2440
JOURNAL Environ. Microbiol. 4 (12), 799-808 (2002)
| Sequence Annotation | Location | Component / Role(s) |
| Start Codon Stop Codon | 1,3 289,291 | start_codon feature/start stop_codon feature/stop |