BBa_K358005

BBa_K358005 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K358005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ken Kajita

Date created: 2010-09-16 11:00:00

Date modified: 2015-05-08 01:12:12

lambda lysis cassette [SRRz]

Details

• Types: DnaRegion
• Roles: engineered_region; Generator

Description

This part is to lead cell lysis. It contains S[holin/anti-holin], R[endolysin] and Rz gene with wt-RBS and double terminator[BBa_B0015].

Notes

We took this gene by PCR from λ -Hind Ⅲ digest, which is the product of TaKaRa Bio. However, there is a mutation on S gene and it's not the wild-type. So, after, we done the point mutation PCR and prepared the wild-type DNA.

Source

λ -Hind Ⅲ digest(product of TaKaRa Bio), BBa_B0015
C. Chang, K. Nam, and R. Young, ???S gene expression and the timing of lysis by bacteriophage lambda,??? J. Bacteriol., vol. 177, Jun. 1995, pp. 3283-3294.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
wt-RBS	2,26	feature/rbs, ribosome_entry_site
antiholin	29,352	sequence_feature, feature/misc
holin	35,352	sequence_feature, feature/misc
endolysin	336,812	sequence_feature, feature/misc
Rz	809,1270	feature/dna, sequence_feature
Rz1	1029,1211	feature/dna, sequence_feature
B0010	1282,1361	feature/BioBrick, engineered_region
B0012	1370,1410	feature/BioBrick, engineered_region
polya	1534,1547	feature/polya, polyA_site
stop	1540,1540	feature/stop, stop_codon
stem_loop	1567,1610	stem_loop, feature/stem_loop
T7 TE	1651,1670	feature/stem_loop, stem_loop
stop	1677,1677	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K358005/1

Member of these Collections

• iGEM Parts Registry