| Types | DnaRegion
|
| Roles | primer
Primer
|
| Sequences | BBa_K360114_sequence (Version 1)
|
Description
As reported in the design notes of part BBa_K216015, its sequencing shows an unexpected insertion of 6 bases, ACCACC, after the scar between the RBS and the ATG of the luciferase coding sequence; that is, the sequence reads ACTAGACCACCATG rather than ACTAGATG. This makes the RBS 12 bases from the ATG, somewhat more than the optimum. This primer intends to reestablish the efficiency.
Notes
We were not able to carry out an appropriate assay to check whether deleting the insertion was more efficient.
Source
This primer is composed by the prefix, the RBS BBa_B0034, and a spacer of 7 nucleotides.
