Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Noah Young
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:13
The consensus calcium dependent response element binding site for the Crz1 activator.
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K363001_sequence (Version 1) |
Description
This part is a binding site for the Crz1 transcriptional activator. When placed upstream of a eukaryotic promoter sequence, this sequence results in the calcium dependant transcription of downstream coding sequences given that Crz1 and the upstream signal transducing phosphotase calcineurin are constitutively expressed. The signal cascade occurs when calcineurin binds to cytosolic calcium, activating its phosphotase activity. The active calcineurin dephosphorylates cytoplasmic Crz1, resulting in translocation of Crz1 to the nucleus where it binds to this part and promotes the assembly of general transcription factors at the promoter sequence.Notes
This part is NOT itself a promoter. It must be inserted upstream of a promoter to function.Source
This part was derived from calcium dependent response element (CDRE) binding sequence of the FSK2 gene. The consensus sequence for Crz1 binding as described by Yoshimoto et al (2002) was mutated at non conserved residues to generate this part. This UAS is the consensus sequence for Crz1 binding, and was used in characterization experiments.| Sequence Annotation | Location | Component / Role(s) |
| Crz1 Binding Site | 1,7 | non_covalent_binding_site feature/binding |