BBa_K368012

BBa_K368012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K368012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2010-10-23 11:00:00

Date modified: 2015-05-08 01:12:15

CMV-promoter+TEV-protease+p14*+TEV recogn. site+eGFP+SV40PA

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K368012_sequence (Version 1)

Description

eukaryots:
CMV-Promoter: konstitutive promoter from cucumber mosaic virus
TEV-Protease: Protesase which cuts at the end of specific recognition sites
p14*: Protein interacting with SF3b155 (Splicing factor 3b, size: 155kDa)
TEV recogn. site: recognition site for TEV-protease
eGFP: eukaryotic codon optimized green fluorescent protein
SV40PA:polyadenylation signal, needed for translation in eukaryots

Notes

-> "Cut'N'Survive" system

Source

biobrick+plasmid+ (Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009)

Components

Access	Instance	Definition
public	BBa_K368010	BBa_K368010
public	BBa_K368011	BBa_K368011

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K368010	1,1927	BBa_K368010
BBa_K368011	1934,2861	BBa_K368011

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K368012/1

Member of these Collections

• iGEM Parts Registry