Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yang Zhang
Date created: 2010-08-26 11:00:00
Date modified: 2015-05-08 01:12:15
pduJK(Propanediol utilization gene J+K)[RBS+pduJ+RBS+pduK]
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K371000_sequence (Version 1) |
Description
Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes. Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduAB consist of pdu A and pdu B wich their natural RBS, which got from Citrobacter Freundii by PCR. PduAB, together with others struture part of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.Notes
The sequence results of pduAB, which we got by PCR from Citrobacter Freundii(NBRC NO.12681), are different from the sequence we found on the papar. We suspect that the strain we used is not same with the papar.Source
pduAb come from Citrobacter Freundii genenom(NBRC NO.12681)| Sequence Annotation | Location | Component / Role(s) |
| pduJ RBS start codon pduJ coding sequence pduK coding sequence stop codon pduK RBS start codon stop codon | 5,12 19,21 19,294 319,783 292,294 307,312 319,321 781,783 | feature/rbs ribosome_entry_site feature/start start_codon CDS feature/cds feature/cds CDS stop_codon feature/stop ribosome_entry_site feature/rbs feature/start start_codon feature/stop stop_codon |