BBa_K371053

BBa_K371053 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K371053
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ruijun Zhu
Date created: 2010-10-26 11:00:00
Date modified: 2015-05-08 01:12:16

theRFC 10 and RFC53 interface converter



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_K371053_sequence (Version 1)

Description

This part locate in BBa_K371051, which is very like pSB1C3 with only a single site nonsense mutation between terminator 0 and chloramphenicol resistance gene. The function of this gene is to convert the any RFC53 part to RFC 10 part without leaving any trace. User will find it is very convenient to make fusion protein with this noval system.

Notes

In the middle of this part is a NheI site, which is designed to examine the correctness of this system.

Source

Since the sequence is not very long, you can easily get it by direct synthesis.

Sequence Annotation Location Component / Role(s)
EarI
BglII
EarI
NheI
SacI
SapI
3,8
28,32
24,29
14,19
1,6
2,8
sequence_feature feature/dna
feature/dna sequence_feature
sequence_feature feature/dna
sequence_feature feature/dna
feature/dna sequence_feature
feature/dna sequence_feature
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K371053/1