BBa_K374005

BBa_K374005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K374005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Patrick Fortuna, Thomas Trolle, Anastasiya S. Haugaard, Martin Malthe Borch, Juliet Frederiksen

Date created: 2010-10-16 11:00:00

Date modified: 2015-05-08 01:12:16

Lambda nutR site - N utilization site + Tr1 rho dependent terminator.

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K374005_sequence (Version 1)

Description

In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element [1]. This part contains the lambda nutR site which together with part (N part #) will suppress termination downstream regardless of what terminator is used.
[1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768.

Notes

Requires a couple hundred base pairs upstream of the terminator.

Source

nutR site from the lambda phage from E.coli.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Rho utilization site A (rutA)	8,25	feature/misc, sequence_feature
N utilization rightward	24,40	feature/misc, sequence_feature
Rho utilization site B (rutB)	41,60	feature/misc, sequence_feature
Rho dependant tR1	77,101	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K374005/1

Member of these Collections

• iGEM Parts Registry