Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lisa Blanc Iversen, Maya Friis Kjaergaard, Annemi Jollmann, Anja Sander and Grzegorz Slodkowicz
Date created: 2010-10-19 11:00:00
Date modified: 2015-05-08 01:12:16
Gifsy1 promoters and Gifsy1 repressor gogR
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K374008_sequence (Version 1) |
Description
Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR.gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli.