Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Johan Nordholm, Andreas Constantinou, Nina Schiller
Date created: 2010-10-22 11:00:00
Date modified: 2015-05-08 01:12:17
N-part TAT cell-penetrating peptide
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K380001_sequence (Version 1) |
Description
This part encodes the cell-penetrating peptide TAT and may be used in N-terminal fusions with full-length proteins to create transduction proteins with the ability to permeate the lipid bilayer of various cell types.Purified full-length TAT fusion proteins expressed in Escherichia coli have been shown to successfully translocate into several human cell types, including all cells found in whole blood, as well as bone marrow stem cells and osteoblasts, while still retaining the fused protein's activity ([http://www.ncbi.nlm.nih.gov/pubmed/9846587 Nagahara et al. 1998). The mechanism for transduction over the bilipid membrane is still a matter of debate, but has been suggested to occur by macropinocytosis, a specialized form of endocytosis.
Notes
This part was designed from the amino acid sequence and optimized for expression in Escherichia coli. Codon usage has been varied for repetitive amino acids.Source
TAT is an 11-amino acid derivative from the Human Immunodeficiency Virus 1 (HIV-1) trans-activating transcriptional activator (Tat) (Green and Loewenstein, 1988; [http://www.ncbi.nlm.nih.gov/pubmed/9846587 Nagahara et al. 1998).| Sequence Annotation | Location | Component / Role(s) |
| TAT Start codon | 1,36 1,3 | CDS feature/cds start_codon feature/start |