Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andreas Constantinou, Emmelie Lidh, Nina Schiller, Johan Nordholm
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:17
Superoxide dismutase 1 protein
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K380007_sequence (Version 1) |
Description
Superoxide dismutase 1 proteinHuman soluble superoxide dismutase 1(SOD1), complete cds (GenBank: AY450286.1). SOD1 is a soluble cytoplasmic protein functional as a homodimer that binds copper and zink ions. SOD1 catalyzes the reaction O-2 + O-2 + 2H+ → H2O2 + O2, protecting the cell from oxidative damage. SOD1 was first cloned and expressed in E. coli by Hallewell ??????et al??????., (1985). Co-expression of SOD1 with the yeast copper chaperon (yCCS) (Ahl ??????et al??????. 2003) yields proteins with higher copper contents, leading to increased activity and more stable proteins.
For the helper yeast copper chaperone (yCCS), see [[Part:BBa_K380008]].
Notes
The start and stop codon was removed and the gene was redesigned with BioBrick prefix and suffix according to assembly standard 25 using specifically designed primers and PCRSource
Homo sapiens superoxide dismutase (SOD) mRNA, complete cds (GeneBank: AY450286.1), 465 nucleotide gene. First reported by Hallewell ??????et al??????., (1985).| Sequence Annotation | Location | Component / Role(s) |
| superoxide dismutase s_d_mut | 1,459 302,302 | CDS feature/cds feature/mutation sequence_alteration |