| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K385005_sequence (Version 1)
|
Description
This part encodes a sequence that is capable of forming a stem loop in the mRNA. Moreover, this stem loop is bound in a sequence and structure-specific manner by the N-peptide sequence (see part numbers BBa_K385003 and BBa_K385004). The mRNA stem sequence is derived from phage lambda, and forms part of a transcriptional termination attenuation system. This stem loop encoding sequence can be used as part of a eukaryote gene expression control strategy. Insertion of this stem loop into the 5' untranslated region (5'UTR) of a target gene (i.e. between the transcript start site and the AUG translation initiation site) will allow this mRNA to be actively translated in the absence of the N-peptide sequence. However, expression of the N-peptide in trans will allow N-peptide binding to the B-box stem, causing translational attenuation by inhibition of ribosome scanning along the 5'UTR.
Notes
The sequence forms part of a 5' untranslated leader, and so must be free from all AUG sequences.
Source
Phage lambda genome
