Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Han Zhu
Date created: 2010-10-14 11:00:00
Date modified: 2015-05-08 01:12:18
MEF2-JeT promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K387011_sequence (Version 1) |
Description
This promoter is an alignment of MEF2 enhancer and JeT core promoter in Eukaryotes, and is used in sensing the calcium signal. MEF2 enhancer element is the binding site of transcription factor MEF2c， which recruits Cabin1 and HDAC (Histone deacetylase) and blocks transcription. The influx of Ca2+ can activates this promoter because calcium and calmodulin could dissociate Cabin1 while p300 HAT (Histone acetyltransferase) competes with Cabin1 for binding to MEF2. (Hong-Duk Youn and Jun O. Liu, 2000)JeT promoter is a synthetic core promoter which recruits basal transcription machinery. Alignment with enhancer element will up or down regulate the activity of this promoter. (Jens Torn??e, Philip Kusk, Teit E. Johansen et al., 2002)
Notes
We invited a BGH polyA sequence in the front of this promoter to attenuate the influence of constitutive promoters in other commercial vectors (because this promoter should be inserted into shuttle plasmids or virus plasmids).Source
This promoter is synthesized de novo using DNA synthesis techniques.| Sequence Annotation | Location | Component / Role(s) |
| BGH polyA MEF2 enhancer JeT core promoter | 1,224 225,299 300,485 | feature/polya polyA_site non_covalent_binding_site feature/binding promoter feature/promoter |