BBa_K387012

BBa_K387012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K387012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Han Zhu
Date created: 2010-10-14 11:00:00
Date modified: 2015-05-08 01:12:18

CRE-JeT promoter



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K387012_sequence (Version 1)

Description

This part is an alignment of CRE (cAMP response element) enhancer and JeT core promoter which is sensitive to calcium signals in eukaryotic cells. CRE is the binding site of CRE-BP transcription factor family and JeT promoter is a synthetic core promoter which recruits basal transcription machinery.

Notes

We invited a BGH polyA sequence in the front of this promoter to attenuate the influence of constitutive promoters in other commercial vectors (because this promoter should be inserted into shuttle plasmids or virus plasmids).

Source

This promoter is synthesized de novo using DNA synthesis techniques

Sequence Annotation Location Component / Role(s)
BGH polyA
CRE enhancer
JeT
1,224
225,302
303,600
feature/polya polyA_site
non_covalent_binding_site feature/binding
promoter feature/promoter
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BBa_K387012/1