BBa_K390003

BBa_K390003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K390003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cody Tramp
Date created: 2010-07-31 11:00:00
Date modified: 2015-05-08 01:12:19

pilA secretion tag for Synechocystis sp. PCC 6803



Types
DnaRegion

Roles
tag

Tag

Sequences BBa_K390003_sequence (Version 1)

Description

This is the secretion tag on the 5' end of the coding sequence of the pilA gene in Synechocystis sp. PCC 6803. The PilA protein is the major secreted protein in this species (Sergeyenko TV and Los D A. 2000. Identification of secreted proteins of the cyanobacterium Synechocystis sp. strain PCC 6803. FEMS Microbiology Letters 193: 213-216).

In Synechocystis sp. PCC 6803, the system of secretion is based on the charge of the N-terminus leader sequence. Positive charges on the N-terminus leader sequence contribute to secretion in a non-sequence-dependent manner (Sergeyenko TV and Los DA. 2003. Cyanobacterial leader peptides for protein secretion. FEMS Microbiology Letters 218: 351-357).

Notes

none

Source

Based on genomic sequence. Part was created by annealing two oligionucleotides together.

Sequence Annotation Location Component / Role(s)
Start Codon
secretion tag - Synechocystis sp. PCC 6803
1,3
1,64
feature/start start_codon
tag feature/tag
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K390003/1