BBa_K390006

BBa_K390006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K390006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cody Tramp

Date created: 2010-07-31 11:00:00

Date modified: 2015-05-08 01:12:19

sigA Type I promoter from Synechocystis sp. PCC 6803

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K390006_sequence (Version 1)

Description

This is the promoter for the sigma factor sigA in Synechocystis sp. PCC 6803. It is self-regulating (controlled by SigA), but has shown to potentially be down-regulated due to changes from heat-shock, darkness, and salt stress. A decrease in SigA protein was observed in a SigC knockout, but not direct proportion to the transcript of SigA (Imamura S and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3:65-87).

As SigA is the housekeeping sigma factor for Synechocystis sp. PCC 6803, this promoter is likely constitutive, with a moderate level of expression (this is pending experimental verification).

Notes

none

Source

Sequence for genomic DNA, part assembled by annealing of two oligionucleotides.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
sigA promoter	1,42	promoter, feature/promoter
-35 box	6,11	feature/misc, sequence_feature
-10 box	27,32	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K390006/1

Member of these Collections

• iGEM Parts Registry