| Types | DnaRegion
|
| Roles | Primer
primer
|
| Sequences | BBa_K391004_sequence (Version 1)
|
Description
S. aureus plasmid replicon. Can allow a BioBrick plasmid to replicate and segregate in S. aureus. Derived from pT181. Can use either plasmids pT181 or plasmids derived from pT181 (e.g. pcN33). Add EcoRI and SpeI sites before using. Expect a band ~2kbp after PCR.
Notes
Note a base pair difference between the forward primer given in the paper and the pT181 gene bank information. UBC iGEM used pT181 sequence information instead.
Source
From Charpentier, et al.. Novel Cassette-Based Shuttle Vector System for Gram-Positive Bacteria. Appl Environ Microbiol. 2004 October; 70(10): 6076???6085. Refer to pT181 gene bank information.
