BBa_K394000

BBa_K394000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K394000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brian Landry

Date created: 2010-10-17 11:00:00

Date modified: 2015-05-08 01:12:21

Resistance Gene for G418 in Yeast

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K394000_sequence (Version 1)

Description

This part confers resistance to Geneticin (G418) in yeast.

Selection of yeast cells is typically preformed on plates with 200??g/ml of G418.

Notes

None

Source

The part was synthesized from the PMKV005 plasmid used in McCusker et. al. The coding region contains the KanR open reading from transposon Tn903 from E. coli.

1. McCusker JH, Vorachek-Warren MK. DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae. Yeast (Chichester, England). 2004;21(2):163-71. Available at: http://www.ncbi.nlm.nih.gov/pubmed/14755641.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start Codon	1,3	start_codon, feature/start
KanR gene	1,810	feature/cds, CDS
HindIII cut site	545,549	feature/misc, sequence_feature
Stop	808,813	stop_codon, feature/stop

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K394000/1

Member of these Collections

• //cds/selectionmarker
• //cds/selectionmarker/antibioticresistance
• //chassis/eukaryote/yeast
• iGEM Parts Registry