BBa_K395600

BBa_K395600 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K395600

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Toshitaka Matsubara

Date created: 2010-10-03 11:00:00

Date modified: 2015-05-08 01:12:21

Apple fragrance enzyme (MpAAT1) generator

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K395600_sequence (Version 1)

Description

BBa_K395600 produce MpAAT1 enzyme that catalyzes production of 2-methylbutyl acetate and butyl acetate from 2-methylbutanol and butanol. 2-methylbutyl acetate and butyl acetate has a apple smell.

Notes

T7 promoter+lac operator+RBS+His tag+thirombin+S・tag+Factor Xa+MpAAT1+His tag

Source

apple

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0185	5,49	feature/BioBrick, engineered_region
T7 promoter sequence	6,22	promoter, feature/promoter
LacI binding site	25,49	feature/binding, non_covalent_binding_site
rbs	80,84	feature/rbs, ribosome_entry_site
start	76,76	feature/start, start_codon
his-tag	97,114	tag, feature/tag
s-tag	148,181	feature/tag, tag
factor-Xa	223,234	non_covalent_binding_site, feature/binding
MpAAT1	235,1602	feature/cds, CDS
stop	1602,1602	stop_codon, feature/stop
His-tag	1681,1698	tag, feature/tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K395600/1

Member of these Collections

• iGEM Parts Registry