Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yumiko Kinoshita
Date created: 2010-10-07 11:00:00
Date modified: 2015-05-08 01:12:22
rbs+crtZW under pBad promoter for double plasmids
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K395706_sequence (Version 1) |
Description
This part contains crtZW, which works as the astaxanthin biosynthesis pathway when add beta-carotene as a substrate.It is necessary to add either beta-carotene or the synthesis operon.
Notes
・This operon is under arabinose inducible promoter.・I used this part with beta-carotene synthesis operon (BBa_K274210) as double plasmids in the project.
Source
CrtZ comes from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae.CrtW comes from Brevundimonas sp. strain SD212 (MBIC 03018) (Accession number AB377271).
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This Composite Biobrick is created by standard assembly of Part BBa_I0500, Part BBa_I742158 and Part BBa_K395705.
| Access | Instance | Definition |
| public public public | BBa_I0500 BBa_I742158 BBa_K395705 | pBad/araC rbs+crtZ BBa_K395705 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 BBa_I742158 BBa_K395705 | 1,1210 1219,1765 1774,2524 | pBad/araC rbs+crtZ BBa_K395705 |