BBa_K396000

BBa_K396000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K396000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ryuji Yamamoto

Date created: 2010-10-20 11:00:00

Date modified: 2015-05-08 01:12:22

T7/CI Hybrid Promoter with GFP (composite with LuxR generator)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K396000_sequence (Version 1)

Description

T7/CI hybrid promoter is the hybrid promoter which is activated by T7 RNA Polymerase and regulated by lambda CI protein.

Notes

LuxR generator functions as it is designed. T7/CI hybrid promoter function is checked by using BL21(DE3) strain, resulted in that it works within limited range of the expression of T7 RNA polymerase (IPTG concentration<100μM).

Source

Lambda CI operator sequence (OR1, BBa_R1051) is connected to T7 Promoter (BBa_I719005).
LuxR generator is consisted of constitutive promoter (BBa_J23100) and LuxR coding sequence (BBa_C0062).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LuxR	4,757	feature/protein, CDS
Constitutive promoter	784,818	feature/promoter, promoter
T7 promoter	833,855	promoter, feature/promoter
Lambda CI (OR1 operator)	855,868	feature/operator, operator
GFP	901,1616	feature/protein, CDS
LVA	1616,1654	feature/tag, tag

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K396000/1

Member of these Collections

• iGEM Parts Registry