Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hugo Federico Cueto Rojas
Date created: 2010-10-11 11:00:00
Date modified: 2015-05-08 01:12:23
Prefoldin-beta generator
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K398403_sequence (Version 1) |
Description
Subunit alpha of the prefoldin from Pyrococcus horikoshii OT3. Four subunits alpha and two subunits beta create a chaperone that confers solvent tolerance to E. coli K12Notes
Enhanced sequence for expression on E. coli K12, restriction sites for the enzymes XbaI, PstI, EcRI and SpeI are removed from the CDS. An extra XbaI site will be found between RBS and Promoter so you can increase the expression level of the protein by changing the promoter.Source
The NCBI genomic sequence was enhanced for iGEM purposes| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_J61107 PhPFD-beta start stop BBa_K398401 XbaI site | 1,35 44,55 62,415 62,64 413,415 62,415 36,41 | feature/BioBrick engineered_region engineered_region feature/BioBrick feature/cds CDS start_codon feature/start feature/stop stop_codon feature/BioBrick engineered_region feature/misc sequence_feature |