Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hugo Federico Cueto Rojas and Ramon van der Valk
Date created: 2010-10-11 11:00:00
Date modified: 2015-05-08 01:12:23
Solvent tolerance cluster
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K398406_sequence (Version 1) |
Description
Four subunits alpha and two subunits beta create a chaperone that confers solvent tolerance to E. coli K12Notes
Enhanced sequence for expression on E. coli K12, restriction sites for the enzymes XbaI, PstI, EcRI and SpeI are removed from the CDS. An extra XbaI site will be found between RBS and Promoter so you can increase the expression level of the protein by changing the promoter.Source
Genomic sequence obtained from NCBI, the sequences were enhanced for iGEM purposes| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_J61107 start PhPFD-alpha stop BBa_K398400 BBa_J61107 PhPFD-beta start stop BBa_K398401 XbaI site | 1,35 44,55 62,64 62,517 515,517 62,517 526,537 544,897 544,546 895,897 544,897 36,41 | feature/BioBrick engineered_region engineered_region feature/BioBrick feature/start start_codon CDS feature/cds feature/stop stop_codon engineered_region feature/BioBrick engineered_region feature/BioBrick feature/cds CDS start_codon feature/start stop_codon feature/stop feature/BioBrick engineered_region feature/misc sequence_feature |