BBa_K400628

BBa_K400628 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K400628
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Seobeom Heo
Date created: 2010-10-18 11:00:00
Date modified: 2015-05-08 01:12:23

mAAA (EcoRI site mutated Aryl Acyl-Amidase)



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K400628_sequence (Version 1)

Description

Aryl acylamidase is an enzyme that acts in the amide bond between aryl- and acyl- compounds.
The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is reversible.
When a p-acetaminophenol was reacted with AAA, the product gave a purple-red color.
As a result, a clone presenting AAA activity from the genomic library gave a color change around the colony when it was grown on solid media enriched with p-acetaminophenol.

Since it gives color to colorless substance, it can be used as a reporter gene to a specific promoter.
In our project, we put it downstream of cadmium-dependent promoter to detect any cadmium present in a solution.

Notes

This part was cloned into pSB1C3 vector and mAAA was mutated in two EcoRI sites.

Source

It was identified from a genomic library of a soil bacterium, which was isolated using a selection media having p-acetaminophenol as a sole carbon source.

Sequence Annotation Location Component / Role(s)
mutated aryl acylamidase
1,1494
feature/protein CDS
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K400628/1