Types | DnaRegion
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Roles | engineered_region
Reporter
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Sequences | BBa_K400628_sequence (Version 1)
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Description
Aryl acylamidase is an enzyme that acts in the amide bond between aryl- and acyl- compounds.
The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is reversible.
When a p-acetaminophenol was reacted with AAA, the product gave a purple-red color.
As a result, a clone presenting AAA activity from the genomic library gave a color change around the colony when it was grown on solid media enriched with p-acetaminophenol.
Since it gives color to colorless substance, it can be used as a reporter gene to a specific promoter.
In our project, we put it downstream of cadmium-dependent promoter to detect any cadmium present in a solution.
Notes
This part was cloned into pSB1C3 vector and mAAA was mutated in two EcoRI sites.
Source
It was identified from a genomic library of a soil bacterium, which was isolated using a selection media having p-acetaminophenol as a sole carbon source.