Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Po-Hsiang Liao
Date created: 2010-10-14 11:00:00
Date modified: 2015-05-08 01:12:25
Theophylline-inducible Riboswitch
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K411001_sequence (Version 1) |
Description
The discovery of the riboswitch was based on data which described conserved RNA secondary structure found on 5???-untranslated regions and the creation of small-molecule binding mRNA, aptamers. The function of these riboswitches is similar to the function of inducible promoters in that they both regulate downstream genetic data: their difference is that while promoters regulate transcription of DNA, riboswitches control translation of RNA.A riboswitch is can be separated into two components: an aptamer and an expression platform. These two components work together to form a ???switch???. The aptamer binds to a small molecule inducer, and the expression platform structurally changes to regulate gene expression.
Notes
We want to choose a kind of riboswitch which has the following characteristics:The inducer cannot metabolize in E. coli.
It does not exist in naturally occurring E. coli
It does not have EcoRI,X,S, or PstI cutting site
Source
ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagforward primer : gaattcgcggccgcttctagag ggtgataccagcatcgtcttgatgcccttggcag
reverse primer : ctgcagcggccgctactagtacttgttgtcttgcagcggggtgctgccaagggcatcaagac
| Sequence Annotation | Location | Component / Role(s) |
| rbs Theophylline binding site (part B) Theophylline binding site (part A) | 46,56 25,30 10,11 | feature/rbs ribosome_entry_site feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding |