BBa_K414001

BBa_K414001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K414001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Elaine Bersaba

Date created: 2010-09-16 11:00:00

Date modified: 2015-05-08 01:12:26

Strong Plant RBS (Kozak sequence) + GFP from E0040

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K414001_sequence (Version 1)

Description

In order for Green Fluorescent Protein to function properly in plants, a plant-specific ribosome binding site (Kozak sequence)must be added to the 5'region just upstream from the start of translation.The Kozak sequence is AAA AAA AAA ACA and is directly adjacent to the ATG start codon in GFP.

Notes

We chose a strong plant Kozak sequence that can function in a broad range of dicot species, including tobacco and Arabidopsis.

Source

The Kozak sequence was directly engineered into the prefix primer and used to PCR out the GFP gene from E0040.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Kpn I	1,6	feature/misc, sequence_feature
Kozak	7,18	feature/rbs, ribosome_entry_site
Translational Start	19,21	feature/start, start_codon
GFP ( E0040 )	19,732	CDS, feature/cds
Translational Stop 2x	733,738	feature/stop, stop_codon

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K414001/1

Member of these Collections

• iGEM Parts Registry