BBa_K414008

BBa_K414008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K414008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elaine Bersaba
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:12:26

rd29A Promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K414008_sequence (Version 1)

Description

rd29A promoter is regulated by cold stress responses, salinity and drought by using alternative ABA independent and dependent transcription factors.

From previous studies, the rd29A promoter not only increases the resistance to different stresses in plants, but it also minimizes the negative effects such as dwarfism (only 30% grown reduction compared to the wild type plant and only a slight growth retardation phenotype on the tobacco plant ) in comparison to the 78% growth reduction of the 35S promoter. This proved that the rd29A is a better promoter than the 35S when used with a stress reporter. Another study also showed that the survival rates of the transgenic clones (with the rd29A promoter) had a greater probability of survival after recovery from exposure to freezing temperatures. This was compared to the non-transgenic cells that showed damage to the plant with no recovery after freezing. Therefore, the rd29A promoter is critically important for it can potentially improve agricultural techniques that farmers can use for their crops.

Notes

The rd29A promoter was isolated by the use of the rd29A designed primers in PCR. This was blunt end Topo cloned, digested with EcoR 1 and Pst 1 sites, and was then ligated to the pSB1C3 vector.

Source

This promoter came from the synthetic design that contained the rd29A Promoter + Strong Plant Kozak (RBS) + RFP in the pMA vector.

Sequence Annotation Location Component / Role(s)
rd29A
1,836
feature/promoter promoter
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K414008/1