| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K415031_sequence (Version 1)
|
Description
This part is a promoter that was designed as a correction to the anomalous BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.
Notes
This promoter was designed entirely as a result of issues with the R0065 promoter, which was essentially designed as a literal fusion of two promoters that happened to contain twin -10 transcription initiation sites. This resulted in a promoter that was not leaky without the presence of AHL, but constitutively on. This newly designed promoter should properly follow the logic detailed above.
Source
This is a hybrid promoter synthesized from a portion of the Vibrio Fischerii Lux operon and the cI/cro switch of Lambda bacteriophage.
