Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2010-06-25 11:00:00
Date modified: 2015-05-08 01:12:27
RBS : pVIII
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K415100_sequence (Version 1) |
Description
A copy of pVIII, a surface protein that is highly expressed by bacteriophage M13. This part has applications in phage display.Notes
This sequence was produced via PCR, and it was determined after a graded PCR experiment that the optimal ligation temperature for pVIII was 60 degrees Celsius.Source
This ~300 bp part was produced via PCR from the genome of bacteriophage M13X07. The primers read as follows:Forward: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGaaagaggagaaatactagATGAAAAAGTCTTTAGTCCTCAAAGCCTC
Reverse: [To be specified]
These primers ensured that the PCR included the standard BioBrick restriction sites.
| Sequence Annotation | Location | Component / Role(s) |
| B0034 Strong RBS pVIII | 1,12 19,240 | feature/rbs ribosome_entry_site CDS feature/cds |