Types | DnaRegion
|
Roles | Translational_Unit
engineered_region
|
Sequences | BBa_K415116_sequence (Version 1)
|
Description
A copy of pIX, an end surface protein that is expressed by bacteriophage M13. pIX is also involved with initiation of M13 assembly. This part has applications in phage display.
Notes
This ~300 bp part was produced via PCR from the genome of bacteriophage M13X07. The primers read as follows:
Forward: [To be specified]
Reverse: [To be specified]
These primers ensured that the PCR included the standard BioBrick restriction sites.
Source
This part originates in bacteriophage M13X07, specifically the 99 base-pair sequence for gIX and a weak ribosome binding site [To be specified].