Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2010-07-10 11:00:00
Date modified: 2015-05-08 01:12:27
RBS Weak : pVIII-Jun
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K415118_sequence (Version 1) |
Description
A modified copy of pVIII, a surface protein that is highly expressed by bacteriophage M13. This particular copy includes a fusion with the Jun protein. This part has applications in phage display, especially when paired with the Fos protein, with which it can form a very stable heterodimer.Notes
The sequence for Jun is extensive at 1005 bp; therefore, designing the sequence as a simple primer is prohibitively costly. We instead constructed the sequence using PCR SOEing; in between the parts is a [pentaglycine linker bridge (GGAGGAGGAGGAGGA) or glycine-serine bridge (GGAGGAGGAGGAAGU).]Source
The source of the weak RBS is part BBa_B0031. The source of the pVIII protein is the genomic sequence of the M13KE bacteriophage, and the Fos protein can be found in the PubMed nucleotide archives.| Sequence Annotation | Location | Component / Role(s) |
| B0031 RBS Weak pVIII (M13KE) Glycine-Serine Linker Jun (Murine) | 1,14 21,242 243,257 258,1262 | ribosome_entry_site feature/rbs CDS feature/cds feature/binding non_covalent_binding_site feature/cds CDS |