Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2010-08-19 11:00:00
Date modified: 2015-05-08 01:12:27
Strong Constitutive T5 Promoter with cmt Operator (T5-cmt)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K415200_sequence (Version 1) |
Description
This is a strong, constitutive partial T5 promoter with the cmt operator appended immediatelyafterward. This construct was specified in a recent (as of 2010) paper by Choi, et al., titled, "A
novel, versatile, and tightly regulated expression system for E. Coli strains." In the absence
of cymR, this promoter should function as a strong, constitutive promoter; in the presence of
cymR, this promoter should be repressed with essentially zero leaky transcription.
Notes
This promoter contains the T5 partial promoter, the sequence of which was derived from the 2010 Choi, et al. paper. Like T7 bacteriophage promoters, this T5 promoter is constitutive and strong; unlike T7 promoters, however, it does not require T7 RNA Polymerase. This means that the T5 promoter could have wide applicability as a strong promoter in Escherichia coli and other organisms.Source
This part was synthesized via minigene construction by Mr. Gene; its source is the 2010 paper by Choi, et al., titled, "A novel, versatile, and tightly regulated expression system for E. Coli strains."| Sequence Annotation | Location | Component / Role(s) |
| cmt Operator T5 Partial Promoter | 54,85 1,53 | feature/operator operator promoter feature/promoter |