BBa_K415202

BBa_K415202 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K415202

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grant Robinson

Date created: 2010-08-19 11:00:00

Date modified: 2015-05-08 01:12:27

cymR Repressor Protein

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K415202_sequence (Version 1)

Description

The cymR repressor tightly and efficiently binds the cmt operator. In conjunction with part
BBa_K415200, this repressor may be used to create an inverter or similar logical circuitry. cymR
changes conformation upon induction with cumate (isopropylbenozate), and may be used in a
manner similar to the lacI/IPTG system.

The cymR repressor appears to bind the complementary cmt operator more efficiently than
lac repressor binds the lac operator, according to Choi, et al. in a recent (as of 2010) paper titled, "A novel, versatile, and tightly regulated expression system for E. Coli strains."

Notes

This repressor required site-directed mutagenesis of nucleotide 132 to a G in order to remove
a PstI cut site.

Source

CymR was derived from the organism Pseudomonas Putida. The copy of cymR used in the construction of BBa_K415202 was generously donated by Noah Davidsohn.

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K415202/1

Member of these Collections

• iGEM Parts Registry