BBa_K415206

BBa_K415206 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K415206
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2010-08-19 11:00:00
Date modified: 2015-05-08 01:12:27

J23117 : B0034 : cymR Repressor Protein



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K415206_sequence (Version 1)

Description

This is a copy of a strongly expressed cymR repressor under the control of a weak, constitutive
promoter. This is a modular component for circuit construction and a relatively strong cymR generator.

Notes

Note that a weak promoter was chosen in order to follow the construction design of Choi, et
al. in the 2010 paper, "A novel, versatile, and tightly regulated expression system for E. Coli
strains." Previous work by Choi and others in M. Extorquens indicated that overexpression
of cymR was potentially toxic to bacterial cells; although data concerning this toxicity is
unavailable, the researchers managed to develop a tightly expressed cymR switch by placing
cymR under the control of a weak, constitutive promoter for kanamycin resistance. As our
team was unable to clarify the origin of this sequence, we instead selected a weak, constitutive
promoter from the Anderson library.

Source

This part is composed of BioBricks BBa_J23117 and BBa_K415204.

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K415206/1