Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM 2010 MIT
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:27
Low Power Toggle - Ptrc:cI:tt:Plambda:LacI:tt
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K415301_sequence (Version 1) |
Description
[[Image:CollinsToggle.png|frame|150px|right|The original pTSMa plasmid from the Collins paper. This plasmid has been modified so that it now contains cI that is hypersensitive to cleavage by RecA = Low Power Toggle.]]This part is a variation of a UV Toggle (2004) from an article in PNAS. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.
This part improves upon its predecessor in that it requires less UV power to switch states, thus killing fewer cells (see E.Coli death curve).
[[Image:Deathcurve.png|frame|150px|right|E.Coli population density as a function of UV exposure. Measured by drip assay.]]
The following data was taken from cells co-transformed with K415301 (with and [[Part:BBa_K415023]].
{|style="border-width: 1px; width: 200px; border-style: solid; border-color: #000; width: 700px; text-align:center;"
!colspan="6"|
UV Power Modulation
|-
|colspan="2"|Photos
(in J/m^2, ignore W)
|colspan="4"|
|-
|colspan="2"|FACS
(in J/m^2)
|colspan="1"|[[Image:]]
|colspan="1"|[[Image:]]
|colspan="1"|[[Image:]]
|colspan="1"|[[Image:]]
|-
|colspan="2"|
|colspan="1"|0
|colspan="1"|5
|colspan="1"|20
|colspan="1"|40
|}
Notes
None.Source
From the [2010 iGEM Team].This part is a variation of a UV Toggle (2004) from an article in PNAS.