Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniela Mehech
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:12:29
FokI Cleavage Domain (fok+)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K426012_sequence (Version 1) |
Description
A similar part exists in the Registry of standard biological parts, but this part sequence differs by 1 mutation and 3 extra AA at N-term.This part encodes a DNA modifying enzyme.
This part is associated with Zinc Finger Nuclease devices.
A zinc finger nuclease is an engineered protein derived from two zinc finger DNA binding proteins and cutting domains usually from a type IIS restriciton enzyme. The special thing about them is that 1) they bind long sequences of DNA, long enough that it might be unique in a eukaryotic genome, and 2) zinc finger proteins that bind to most sequences can be generated somewhat easily. So, you can use a zinc finger nuclease to introduce a ds break at a specific user-defined site in a eukaryotic genome if the protein is delivered to the nucleus of the cell. Eukaryotic ds breaks stimulate homologous recombination. So, if you deliver both the ZFN and a DNA homologous to the target site, you get insertion of your DNA into that site of the genome.