BBa_K427001

BBa_K427001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K427001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jan Marte Contreras Ortiz
Date created: 2010-10-21 11:00:00
Date modified: 2015-05-08 01:12:29

C protein of the Mu bacteriophage



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K427001_sequence (Version 1)

Description

C is a protein of the Mu bacteriophage. It is typically transcribed by the middle promoter of the phage (Pm) and it activates the four late promoters Plys, Pi, Pp and Pmom. Since it is the activator of the late it can be used alongside them to build a sensitivity tuner that increases the POPS output of a construct. The part includes the ribosome binding site, so it can be used directly after a promoter in a genetic construct.

Notes

The sequence was optimized for its production on E. coli and the ribosome binding site was added at the beginning of the sequence.

Source

Synthetized from the reported sequence of C protein from Mu bacteriophage. It was optimized for its expression in E. coli.

Sequence Annotation Location Component / Role(s)
RBS
MuC
1,12
18,438
feature/rbs ribosome_entry_site
feature/protein CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K427001/1