BBa_K427002

BBa_K427002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K427002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jan Marte Contreras Ortiz
Date created: 2010-10-21 11:00:00
Date modified: 2015-05-08 01:12:29

Mor protein of Mu bacteriophage



Types
DnaRegion

Roles
engineered_region

Translational_Unit

Sequences BBa_K427002_sequence (Version 1)

Description

Mor is a protein of the Mu bacteriophage. It is typically transcribed by the early promoter of the phage and it activates the middle promoter Pm. Since it is the activator of Pm it can be used alongside this promoter to build a sensitivity tuner that increases the POPS output of a construct. The part includes the ribosome binding site, so it can be used directly after a promoter in a genetic construct.

Notes

We did an E. coli codon optimization of the sequence and added a ribosome binding site to the sequence before synthesis.

Source

Synthetized from the reported sequence of Mor protein from Mu bacteriophage. It was optimized for its expression in E. coli.

Sequence Annotation Location Component / Role(s)
RBS
MuMor
1,12
19,405
feature/rbs ribosome_entry_site
CDS feature/protein
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K427002/1