Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Angie Calderon
Date created: 2010-09-17 11:00:00
Date modified: 2015-05-08 01:12:29
pTEF ( Translation elongation factor promoter)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K431008_sequence (Version 1) |
Description
According to research done on this part it has been determined that when comparing glyceraldehydes-3-phosphate dehydrogenase promoter (pGAP) with translational elongation factor promoter (pTEF), pTEF has similar or even greater expression levels of recombinant protein. It was also determined that pTEF does not require an inducer and it can be used in the mass production of heterologuous proteins.Notes
In order to determine the appropriate assembly standard that the part can be used with it was necessary to check the sequence for each of the restriction enzymes.Source
This promoter sequence was isolated from yeast Pichia pastoris.Appl Microbiol Biotechnol. 2007 Mar;74(3):601-8. Epub 2006 Nov 24.
GenBank no. EF014948 http://www.ncbi.nlm.nih.gov/nuccore/116293730
| Sequence Annotation | Location | Component / Role(s) |
| TEF promoter | 1,424 | promoter feature/promoter |